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- 详细信息
- 技术资料
- 英文名:
5-(and-6)-Carboxyfluorescein (5-(and-6)-FAM, mixed isomer)
- 供应商:
Biotium
- 保存条件:
4°C
羧基荧光素的pKa为6.5,被用作pH指示剂。激发光谱以及对pH的荧光反应类似于BCECF。羧基荧光素可用作示踪染料。染料是膜不透性的,可通过显微注射等方式进入细胞。
5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator. Its excitation spectrum and fluorescence response to pH are similar to those of BCECF. Carboxyfluorescein can also be used as a tracer dye. The dye is membrane-impermeant and can be loaded into cells by microinjection or scrape loading.
- λEx/λEm (pH 9) = 492/514 nm
- ε (pH 9) = 82,000
- Yellow orange solid soluble in pH>6 water
- Store at 4°C and protect from light, especially in solution
- C21H12O7
- MW: 376
- [72088-94-9]
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5-(and-6)-羧基荧光素 Biotium 51013
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