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文献和实验Probing and Stripping of Western Blot
). Incubate with gentle shaking for 60 min. at room temp. or overnight at 4 degrees (after shaking a few minutes at room temp, the membrane can be left in the fridge overnight without shaking). This step blocks the membrane to prevent non specific sticking
can be done overnight at RT with constant agitation. 3) Washed stripped membrane at least 3x for 10min each with PBST, then re-block (in PBST+NFDM or PBST+BSA) overnight at 4oC. Stripping Buffer: 62.5mM Tris pH 6.8 2% SDS 100mM b-ME Quick Mix
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation&
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques. William Telford. Louis E. King
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