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- AMRESCO
- a-shr-3027
- 2025年07月13日
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- CAS号：
51805-45-9
- 规格：
10G
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文献和实验蛋白复合体直接酶消化法Direct Enzymatic Digestion of Protein Complexes
), 20% Tris (2-carboxyethyl)-phosphine hydrochloride (TCEP), 100 mM Iodoacetamide (IAA), 500 mM, prepare fresh Tris-HCl (or Trizma base), 100 mM (pH 8.0) Urea, 8 M in 100 mM Tris-HCl (pH
for this purpose. ::Chaotrophe: urea and thiourea::Reductant: DTT, Tris(2-carboxyethyl) phosphine (TCEP), tributyl phosphine (TBP)::Detergent: CHAPS, triton x-100::Ampholytes::Protease inhibitors Some researchers also add tris buffer.Chaotrophe.Urea
buffers we use are listed below, obviously there are other reductants and detergents that can be used for this purpose.:: Chaotrophe: urea and thiourea:: Reductant: DTT, Tris(2-carboxyethyl) phosphine (TCEP), tributyl phosphine (TBP):: Detergent
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