Human Adiponectin

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 226 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells (Adipokine). The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.

The Acrp30 Human protein contains 226 amino acids and having a molecular mass of 24.54 kDa. Native adiponectin is isolated from human pooled sera and contains all HMW, MMW and LMW fractions.

Human pooled serum

Acrp30 Human was filtered (0.04µm) and lyophilized from 0.5mg/ml supplied in 20mM TRIS, 50mM NaCl and 1mM CaCl2, pH 7.5.

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.

Store lyophilized Acrp30 Human at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Acrp30 Human can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Greater than 90% as determined by SDS-PAGE.

SDS

ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHITVYM KDVKVSLFKK DKAMLFTYDQ YQENNVDQAS GSVLLHLEVG DQVWLQVYGE GERNGLYADN DNDSTFTGFL LYHDTN.

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