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文献和实验THAWING CELLS Remove a vial of Passage 2 Primary Mouse Embryo Fibroblasts (PMEF'S), at a concentration of 3 x 106 from liquid nitrogen and thaw quickly at 37oC. Add the 1ml of cell containing media to 9ml of warmed PMEF media
FEEDER PREPARATION BY GAMMA IRRADIATION
ml tubes per every 50 ml centrifuge tube. 10. Cells are now ready to be gamma irradiated. You need to administer 6,000 rads. If you have not use the Irradiator, you need to ask Sandra, Sukeshi or Torrye. DO NOT USE THE IRRADIATOR
Exposing gels and plates containing radioactive samples to X-ray film
maps and prep gels. Terminology Autoradiography is the direct exposure of film by beta particles or gamma rays. Fluorography is the exposure of film by secondary light that was generated by the excitation of a fluor
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