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文献和实验These protocols should yield enough cytosol and organelles for 1-200 MT/Organelle motility assays. Solutions and Reagents • Freshly removed or flash frozen rat liver • PBS •Homogenization Buffer •Homogenization Buffer containing 0.5 mM mM
Preparation of Mitochondria from Rat Liver
Preparation of Mitochondria from Rat Liver Rat liver is an ideal source for functional intact mitochondria for a number of reasons. We use Sprague-Dawley albino male rats for our studies. Female rats can be used, however for serious studies
Isolation of RNA from Difficult Tissues
. Therefore, care should be taken during the initial preparation of the homogenate to ensure intact total RNA. Brain and Plant Tissues: Protein and Lipid-rich Tissues Isolating total RNA from rat or mouse brain can be very rewarding
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