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文献和实验Fusion of Mouse, Rat, or Hamster Cell
at 800 rpm, 5 minutes, RT. Discard the supernatant. Resuspend the pellet in HAT medium to 5 x 105 cells/ml. Plate 200 ml/well to 96 well plates. Incubate at 37o C, 8-10% CO2
R10 media (= RPMI 1640 + 10% FBS) PBS + 2% EDTA Triton X-100 solution (10% in dH2 O) Effector cell preparation: The mouse NK-cell line KY1 is used as an effector cell. During recovery (e.g. after freezing), these cells
Count cells and resuspend in complete RPMI-1640 at 106 /ml. Note: This concentration of spleen cells gives a good response. If experimental conditions require, a titration of cell concentrations (2-3 x 106 /ml to 105 /ml) should be performed
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