Hoechst 33258 analog 6,129244-

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务。

Hoechst 33258 analog 6

CAS No. : 129244-66-2

MCE 国际站：Hoechst 33258 analog 6

产品活性：Hoechst 33258 analog 6 是 Hoechst 系列的标记染料。Hoechst 系列是活细胞核标记染料。Hoechst 通过结合 DNA 双链中的小沟而结合核酸。Hoechst 倾向于结合富含 A/T 的 DNA 链。同时，富含 A/T 的双链 DNA 使荧光强度显著增强。Hoechst 可穿过细胞膜，结合活细胞或固定细胞。Hoechst 染料的荧光强度随着溶液 pH 升高而增强。

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: General Protocol
Preparation of Hoechst working solution
1.1 Preparation of the stock solution
Dissolve 10 mg of in 5 mL DMSO
Note: It is recommended to store the stock solution at 4℃ or -20℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Hoechst working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain final concentration 10 μg/mL Hoechst working solution.
Note: Please adjust the concentration of Hoechst working solution according to the actual situation.
1.Cell staining
2.1 Suspension cells（6-well plate）
a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×10⁶/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes.
c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

Precautions
1. Please adjust the concentration of Hoechst working solution according to the actual situation.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.

相关产品：MitoSOX Red  |  BODIPY 493/503  |  Nile Red  |  D-Luciferin potassium  |  JC-1  |  FITC  |  Propidium Iodide  |  CFDA-SE  |  FITC-Dextran (MW 4000)  |  Dihydroethidium  |  DiR  |  Calcein-AM  |  Thiazolyl Blue  |  2-NBDG  |  DiI  |  Fluo-4 AM  |  TMRE  |  Cy5.5  |  Indocyanine green  |  Acridine Orange hydrochloride  |  CY5  |  BODIPY 581/591 C11  |  FM4-64  |  CY5-SE  |  PKH 26  |  DiD perchlorate  |  Diphenylterazine

热门产品线：重组蛋白  |  化合物库  |  天然产物  |  荧光染料  |  PROTAC  |  同位素标记物

Trending products：Recombinant Proteins  |  Bioactive Screening Libraries  |  Natural Products  |  Dye Reagents  |  PROTAC  |  Isotope-Labeled Compounds