NucView 488和MitoView 633凋亡检测试剂

试剂盒包含NucView 488 caspase-3 底物和 MitoView 633 线粒体染料。基于caspase-3活性和线粒体膜电位变化，使用荧光显微镜或流式细胞仪分析凋亡细胞。

This kit contains NucView® 488 Caspase-3 Substrate to detect apoptotic cells and MitoView™ 633 mitochondrial membrane potential dye.

• Rapid, no-wash, endpoint or real-time assay
• Non-toxic, allowing multi-day experiments to be performed
• For flow cytometry, microscopy or live cell imaging systems
• Dual detection of caspase activity (green) and mitochondrial membrane potential (red/far-red)

NucView® 488 Caspase-3 Substrate is able to detect both intracellular caspase-3 and at the same time stain the cell nucleus, which is known to undergo morphological changes during apoptosis. MitoView™ 633 is a far-red fluorescent mitochondrial dye. The staining is dependent upon the mitochondrial membrane potential; thus, apoptotic cells exhibit a much lower MitoView™ 633 dye staining compared to healthy cells. Furthermore, the spectral separation of these two dyes minimizes fluorescence overlap. See a video of NucView® & MitoView™ in real-time imaging.

Note: The optimal detection settings for MitoView™ 633 are the same as for Cy®5 and other far-red dyes. However, the dye also has visible red fluorescence and can be imaged using Cy®3 settings as well. As a consequence, the dye cannot be used for two-color imaging with other red probes.

To learn about the advantages of monitoring apoptosis using NucView® caspase-3 substrates, visit the NucView® Technology Page.

NucView is a registered trademark of Biotium. NucView enzyme substrate technology is covered by U.S. patents. Cy dye is a registered trademark of GE Healthcare.