WNT信号靶标PCR芯片WNT Signaling Targ

WNT信号靶标PCR芯片可用于研究WNT信号转导的84个关键应答基因的表达。WNT家族的分泌型生长因子不仅参与细胞功能和极化发育过程的调控，同样对普通的细胞生命活动例如动态平衡和细胞周期也起到调控作用。在细胞中有19个WNT的配体，与Frizzled (FZD)家族受体和辅助受体LRP5和LRP6相结合。WNT信号传导包含了3个通路：经典通路，平面细胞极性通路(PCP)和一个钙离子依赖型通路。其中已得到充分研究和诠释的经典WNT通路通过ß-catenin传导信号来调节细胞周期，细胞生长和增殖，该信号转导通路的失调与癌症和发育类疾病是相关联的。该芯片包含了WNT经典信号通路的转录因子和多研究验证得到的高关联性靶标基因，通过该芯片得到的结果可用于WNT信号传导的激活和抑制性研究。通过实时定量PCR，研究者可以简单可靠地同时检测WNT调节的细胞活动相关基因的表达。
Development & Differentiation: Growth Factors: ANGPTL4, BMP4, CTGF, FGF20, FGF4, FGF9, GDF5 (CDMP-1), GDNF, IGF1, IGF2, IL6, JAG1, TGFB3, VEGFA. Transcription Factors: CDKN2A (p16INK4), EGR1, NANOG, POU5F1 (OCT4), PPARD, RUNX2, SIX1, SOX2, SOX9, T (Brachyury). Other Genes: ANTXR1, CCND1, CCND2, CDH1 (E-cadherin), CDON, DAB2, DLK1, EFNB1, EGFR, FN1, FSTGJA1, ID2, MET, MMP9 (Gelatinase B), NRCAM, NRP1, NTRK2, PDGFRA, SMO, TWIST1. Calcium Binding and Signaling: BGLAP, CACNA2D3, CCND1, CUBN, EGFR, LRP1, MMP2 (Gelatinase A), MMP7 (Matrilysin), MMP9 (Gelatinase B), PTGS2 (COX-2), WNT3A, WNT5A, WNT9A. Adhesion: ABCB1 (MDR-1), BGLAP, CD44, CDH1 (E-cadherin), CTGF, EGFR, FGF4, NRCAM, SOX9. Migration: CD44, CTGF, EFNB1, EGFR, FGF7, FN1, GDNF, IGF1, IL6, IRS1, JAG1, MMP9 (Gelatinase B), NRCAM, NRP1, PDGFRA, PPAP2B, PPARD, SIX1, SMO, TWIST1, VEGFA. Cell Cycle: AHR, BIRC5, CCND1, CCND2, CDKN2A (p16INK4), EGFR, FOSL1 (FRA-1), ID2, IGF1, IGF2, MYC, PTGS2 (COX-2), SOX2. Proteolysis: DPP10, MMP2 (Gelatinase A), MMP7 (Matrilysin), MMP9 (Gelatinase B), PLAUR (uPAR). Signal Transduction: WNT Signaling: AXIN2, BTRC (b-TrCP), DKK1, FGF9, FZD7, LEF1, LRP1, PPAP2B, SFRP2, TLE1, WISP1, WISP2, WNT3A, WNT5A, WNT9A. TGFß Signaling: BMP4, FST, GDF5 (CDMP-1), ID2, TGFB3. Hedgehog Signaling: SMO, PTCH1. Notch Signaling: JAG1. Transcription Factors: WNT Signaling: LEF1, TCF7, TCF7L1, TCF7L2. Others: AHR, CDKN2A (p16INK4), CEBPD, EGR1, ETS2, FOSL1 (FRA-1), KLF5 (GC Box BP), MYC, NANOG, PITX2, POU5F1 (OCT4), PPARD, RUNX2, SIX1, SOX2, SOX9, T (Brachyury), TCF4.