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PCR芯片
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SABio
| Product | Species | Technology | Cat. No. |
| DNA Damage Signaling Pathway PCR Array | Human | Gene Expression | PAHS-029Z |
| DNA Damage Signaling Pathway PCR Array | Mouse | Gene Expression | PAMM-029Z |
| DNA Damage Signaling Pathway PCR Array | Rat | Gene Expression | PARN-029Z |
ATM/ATR Signaling: ATM, ATR, ATRIP, BARD1, BRCA1, CDC25A, CHEK1, CHEK2 (RAD53), CSNK2A2, FANCD2, H2AFX, HUS1, MDC1, PARP1 (ADPRT1), RAD1, RAD17, RAD50, RAD9A, RBBP8, RNF168, RNF8, SMC1A, TOPBP1, TP53. DNA Repair: Nucleotide Excision Repair: CDK7, DDB1, DDB2, ERCC1, ERCC2 (XPD), LIG1, NTHL1, OGG1, PCNA, PNKP, RPA1, SIRT1, TP53, XPA, XPC. Base-Excision Repair: APEX1, FEN1, LIG1, MBD4, MPG, NTHL1, OGG1, PARP1 (ADPRT1), PCNA, TP53, UNG, XRCC1. Mismatch Repair: ABL1, EXO1, MLH1, MLH3, MSH2, MSH3, PCNA, PMS1, PMS2, TP73. Double Strand Break Repair: ATM, BLM, BRCA1, CHEK1, H2AFX, HUS1, LIG1, MDC1, MLH1, MRE11A, NBN (NBS1), PRKDC, RAD50, RAD51, RPA1, TP53BP1, XRCC2, XRCC6 (G22P1). Other Genes: ATR, ATRIP, ATRX, BARD1, BRIP1, CHEK2 (RAD53), CIB1, CRY1, FANCA, FANCD2, FANCG, GADD45A, GADD45G, RAD1, RAD17, RAD18, RAD21, RAD51B, RAD9A, RBBP8, REV1, RNF168, RNF8, SMC1A, SUMO1, TOPBP1, XRCC3. Apoptosis: ABL1, ATM, BARD1, BAX, BBC3, BRCA1, CDKN1A (p21CIP1/WAF1), CHEK2 (RAD53), CIB1, CSNK2A2, PPP1R15A (GADD34), PRKDC, RAD21, RAD9A, SIRT1, TP53, TP73. Cell Cycle: ATM, ATR, ATRIP, CDC25A, CDC25C, CDK7, CDKN1A (p21CIP1/WAF1), CHEK1, CHEK2 (RAD53), DDIT3 (GADD153/CHOP), MAPK12, MCPH1, MDC1, PPM1D, PPP1R15A (GADD34), TP53, TP73.
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文献和实验（GDC），其中固定的引物能特异性的检测非转录的基因组DNA重复片段，从而检测样品中是否存在DNA污染。H7到H9是三个重复的孔，均为反转录参照（RTC），用于检测RT反应的效率。H10到H12是阳性PCR参照（PPC），反映了PCR反应的效率。这些孔中加入了人工合成的DNA序列和相应的引物对。两组重复的对照RTC和PPC也可以用于检测芯片间和芯片内的一致性。 预设计的信号通路特异性PCR芯片 96孔模式的RT2Profiler PCR芯片根据SuperArray的Pathway-Centric
Measuring the Formation and Repair of DNA Damage by Ligation-Mediated PCR
). I describe the technical details of LM-PCR as exemplified by the mapping of DNA damage products in ultraviolet (UV) light-irradiated cells.
This chapter was written as a guide to using the long-amplicon quantitative PCR (QPCR) assay for the measurement of DNA damage in mammalian as well as nonmammalian species such as Caenorhabditis elegans (nematodes), Drosophila melanogaster
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