未折叠蛋白应答PCR芯片Unfolded Protein R

未折叠蛋白应答增强版PCR芯片可以检测84个在未折叠蛋白应答反应中发挥关键作用的基因，这些基因能识别堆积于内质网中的错误折叠蛋白并对其做出应答反应。此外，这款芯片还可用来确定实验样品中的未折叠蛋白应激信号通路活性是否增加，或者发生变化。结合在内质网中未折叠蛋白上的分子伴侣能激发蛋白激酶级联信号，进而迅速抑制内质网蛋白翻译，使内质网转运反向，激活内质网特异性的泛素酶，在极端压力下甚至可以诱导细胞凋亡。这些信号还可以激活内切核酸酶，从而将特定的成熟胞浆mRNA加工成异构体。这些mRNA异构体可以进一步翻译成活跃的转录因子，从而增加热休克蛋白，蛋白二硫键异构酶，甚至更多分子伴侣蛋白的表达。这条信号通路还包含了能够介导内质网蛋白折叠质控的蛋白糖基化酶，能够识别内质网中胆固醇合成紊乱的传感蛋白，以及可以对胆固醇合成紊乱做出应激反应的转录因子。这款芯片还包含了16个经实验证实的分子标志物基因，通过这16个基因的表达数据以及特定的分类算法，可以计算样品中该信号通路的活性分值。每张芯片中还包含了一系列内参，以便于客户通过ΔΔCT法对数据进行相对定量分析，评估逆转录和PCR的效率，判断是否存在基因组DNA污染。通过实时定量PCR方法，研究者可以方便并且可信地判断未折叠蛋白应答通路的活性，并对相关基因的表达进行集中的分析。

Product	Species	Technology	Cat. No.
Unfolded Protein Response Plus PCR Array	Human	Gene Expression	PAHS-089Y
Unfolded Protein Response PCR Array	Human	Gene Expression	PAHS-089Z
Unfolded Protein Response PCR Array	Mouse	Gene Expression	PAMM-089Z
Unfolded Protein Response PCR Array	Rat	Gene Expression	PARN-089Z
Unfolded Protein Response PCR Array	Chinese Hamster	Gene Expression	PAJJ-089Z

Unfolded Protein Binding: CALR, CANX, CCT4, DNAJB9, DNAJC10, ERO1LB, HSPA1B, HSPA2, HTRA2, PFDN5, PPIA, SCAP, SEC63, SIL1, TCP1, TOR1A, UGGT1. ER Protein Folding Quality Control: EDEM1, GANAB, GANC, PRKCSH, RPN1, SERP1, ERP44, UGGT1. Regulation of Cholesterol Metabolism: INSIG2, MBTPS1, MBTPS2, SCAP, SREBF1. Regulation of Translation: EIF2A, EIF2AK3, PPP1R15A, SERP1. ER Associated Degradation (ERAD): AMFR, DERL1, EDEM1, FBXO6, HERPUD1, HTRA2, HTRA4, MBTPS1, MBTPS2, NPLOC4, NUCB1, OS9, SEL1L, SELS, SYVN1, SEC62, UBXN4, VCP. Ubiquitination: AMFR, HERPUD1, RNF5, SEL1L, SEC62, UBE2G2, UBXN4, UFD1L, USP14, VCP. Transcription Factors: ATF4, ATF6, ATXN3, CALR, CEBPB, CREB3, CREB3L3, ATF6B, DDIT3, ERN1, ERN2, MBTPS1, PFDN5, SCAP, SREBF1, XBP1. Protein Folding: CALR, CANX, CCT4, DNAJB9, DNAJC10, ERO1L, HSPA4L, PFDN5, PPIA, SEC63, SIL1, TCP1, TOR1A, ERP44, UGGT1. Protein Disulfide Isomerization: DDIT3, DNAJC10, ERO1L, ERO1LB, PDIA3, SELS, SREBF1, ERP44. Heat Shock Proteins: DNAJB9, DNAJC10, DNAJC3, HSPA4, HSPA5, HSPH1, SEC63. Apoptosis: MANF, ATXN3, BAX, CALR, CEBPB, DDIT3, EIF2AK3, ERN1, ERN2, HSPA1B, HTRA2, MAPK8, MAPK9, PDIA3, PPP1R15A, SELS, VCP. Pathway Activity Signature Genes: ADM2, ASNS, BEX2, DDIT3, DNAJB9, GINS2, HERPUD1, INHBE, KCNMB3, MCM4, PCNA, RRM2, SLC17A2, TRIB3, TYMS, UHRF1.