TGFß / BMP信号通路PCR 芯片TGFß / BMP

TGFß/BMP信号通路PCR芯片PCR芯片可用于研究TGFß/BMP介导的信号转导相关的84个基因的表达，同时测定实验组样本中TGF信号通路活性是被激活，抑制还是没有变化。该芯片涵盖了TGFß超级家族的细胞因子和受体。SMAD和SMAD靶标基因，关联基因包括粘连因子、胞外信号分子以及转录因子都包含其中。一些下游细胞活动和发育过程相关的基因也包含其中。该芯片同时包含了16个通过实验验证得到的显著标志基因，通过分级运算程序可计算得到活性分值。每块芯片上都设定了一组对照通过ΔΔCT方法分别对反转效率，基因组DNA含量和PCR效率进行评估。通过实时定量PCR，使用者通过实时定量PCR，使用者即能够利用该芯片简单可靠地测定TGF信号通路活性并同时检测TGFß/BMP介导的信号转导相关基因的表达。
TGFβ Superfamily Ligands: Activin: INHA. BMP: AMH, BMP1, BMP2, BMP3, BMP4, BMP5, BMP6, BMP7, GDF2 (BMP9), GDF3 (VGR-2), GDF5 (CDMP-1), GDF6, GDF7. Nodal: NODAL. TGFβ: TGFB1, TGFB2, TGFB3. Inhibitor/Cofactors: BAMBI, CHRD, DCN, LEFTY1, LTBP1, NOG, TGFBI, THBS1, TGIF1. TGFβ Superfamily Receptors: Activin: ACVR1 (ALK2), ACVR2A, ACVRL1 (ALK1). BMP: AMHR2, BMPR1A (ALK3), BMPR1B (ALK6), BMPR2. TGFβ: TGFBR1, TGFBR2, TGFBR3. Transcription Factors and Regulators: GSC, ID1, JUNB, MYC, SMAD1 (MADH1), SMAD2 (MADH2), SMAD3 (MADH3), SMAD4 (MADH4), SMAD5 (MADH5), SMAD7, SOX4, STAT1, TGFB1I1. SMAD Target Genes: TGFβ/Activin-Responsive: ATF4, CDKN1A (p21WAF1/p21CIP1), CDKN1B (p27KIP1), CDKN2B (p15INK2B), COL1A1, COL1A2, DCN, EMP1, GADD45B, GSC, HERPUD1, IFRD1, IGF1, IGFBP3, IL6, JUNB, MYC, PDGFB, SERPINE1 (PAI-1), TGFB1I1, TNFSF10, TGFBI, TGIF1. BMP-Responsive: BGLAP (Osteocalcin), ID1, ID2, JUNB, SOX4, STAT1. Molecules Regulating Signaling of the TGFβ Superfamily: BAMBI, BMPER, CDKN2B (p15INK2B), CHRD, ENG (Evi-1), NOG, PLAU (uPA), SMURF1. Adhesion and Extracellular Molecules: Adhesion Molecules: BGLAP (Osteocalcin), ENG (Evi-1), TGFB1I1, TGFBI. Extracellular Matrix Structural Constituents: BGLAP (Osteocalcin), COL1A1, COL1A2, LTBP1, TGFBI. Genes Involving in Cellular and Developmental Processes: Apoptosis: CDKN1A (p21WAF1/p21CIP1), CDKN1B (p27KIP1), IGFBP3, INHA, STAT1, TGFB1. Embryonic Development: BMP4, SMAD3, SMURF1. Muscle Development: DCN, IGF1, SMAD3. Neurogenesis: INHA, NOG. Reproduction: AMH, AMHR2, INHA. Skeletal Development: BGLAP (Osteocalcin), BMP1, BMP2, BMP3, BMP4, BMP5, BMP6, BMP7, BMPR2, CHRD, COL1A1, COL1A2, IGF1, INHA, NOG. Pathway Activity Signature Genes: ACTC1, BMPR2, EGR2, FAS, FGF2, FSTL3, JUNB, KANK4, KLHL24, NOV, PMEPA1, SERPINE1, SMAD6, SOX4, STK38L, UBASH3B