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PCR芯片
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SABio
TGFβ Superfamily Ligands: Activin: INHA. BMP: AMH, BMP1, BMP2, BMP3, BMP4, BMP5, BMP6, BMP7, GDF2 (BMP9), GDF3 (VGR-2), GDF5 (CDMP-1), GDF6, GDF7. Nodal: NODAL. TGFβ: TGFB1, TGFB2, TGFB3. Inhibitor/Cofactors: BAMBI, CHRD, DCN, LEFTY1, LTBP1, NOG, TGFBI, THBS1, TGIF1. TGFβ Superfamily Receptors: Activin: ACVR1 (ALK2), ACVR2A, ACVRL1 (ALK1). BMP: AMHR2, BMPR1A (ALK3), BMPR1B (ALK6), BMPR2. TGFβ: TGFBR1, TGFBR2, TGFBR3. Transcription Factors and Regulators: GSC, ID1, JUNB, MYC, SMAD1 (MADH1), SMAD2 (MADH2), SMAD3 (MADH3), SMAD4 (MADH4), SMAD5 (MADH5), SMAD7, SOX4, STAT1, TGFB1I1. SMAD Target Genes: TGFβ/Activin-Responsive: ATF4, CDKN1A (p21WAF1/p21CIP1), CDKN1B (p27KIP1), CDKN2B (p15INK2B), COL1A1, COL1A2, DCN, EMP1, GADD45B, GSC, HERPUD1, IFRD1, IGF1, IGFBP3, IL6, JUNB, MYC, PDGFB, SERPINE1 (PAI-1), TGFB1I1, TNFSF10, TGFBI, TGIF1. BMP-Responsive: BGLAP (Osteocalcin), ID1, ID2, JUNB, SOX4, STAT1. Molecules Regulating Signaling of the TGFβ Superfamily: BAMBI, BMPER, CDKN2B (p15INK2B), CHRD, ENG (Evi-1), NOG, PLAU (uPA), SMURF1. Adhesion and Extracellular Molecules: Adhesion Molecules: BGLAP (Osteocalcin), ENG (Evi-1), TGFB1I1, TGFBI. Extracellular Matrix Structural Constituents: BGLAP (Osteocalcin), COL1A1, COL1A2, LTBP1, TGFBI. Genes Involving in Cellular and Developmental Processes: Apoptosis: CDKN1A (p21WAF1/p21CIP1), CDKN1B (p27KIP1), IGFBP3, INHA, STAT1, TGFB1. Embryonic Development: BMP4, SMAD3, SMURF1. Muscle Development: DCN, IGF1, SMAD3. Neurogenesis: INHA, NOG. Reproduction: AMH, AMHR2, INHA. Skeletal Development: BGLAP (Osteocalcin), BMP1, BMP2, BMP3, BMP4, BMP5, BMP6, BMP7, BMPR2, CHRD, COL1A1, COL1A2, IGF1, INHA, NOG. Pathway Activity Signature Genes: ACTC1, BMPR2, EGR2, FAS, FGF2, FSTL3, JUNB, KANK4, KLHL24, NOV, PMEPA1, SERPINE1, SMAD6, SOX4, STK38L, UBASH3B
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文献和实验（GDC），其中固定的引物能特异性的检测非转录的基因组DNA重复片段，从而检测样品中是否存在DNA污染。H7到H9是三个重复的孔，均为反转录参照（RTC），用于检测RT反应的效率。H10到H12是阳性PCR参照（PPC），反映了PCR反应的效率。这些孔中加入了人工合成的DNA序列和相应的引物对。两组重复的对照RTC和PPC也可以用于检测芯片间和芯片内的一致性。 预设计的信号通路特异性PCR芯片 96孔模式的RT2Profiler PCR芯片根据SuperArray的Pathway-Centric
Directing Human Embryonic Stem Cells to a Retinal Fate
a protocol for directing human embryonic stem cells to generate retinal progenitors and various types of retinal neurons. Our method uses a multistep protocol in which embryoid bodies are treated with inhibitors of both BMP signaling and canonical Wnt
interferon response elements in the promoter region, which are required for binding of the interferon-stimulated gene factor 3 (ISGF3) complex and for janus kinase/signal transducer of transcription (JAK/STAT)-pathway-mediated signaling. Expression of PRGs
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