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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
SMARTer PCR cDNA Synthesis Kit
- 供应商:
北京智杰方远
- 保存条件:
-20度
- 规格:
10 次
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文献和实验Inverse PCR & Cycle Sequencing
2 units Taq 0.4ul Primers for PCR : type of P element 5' or 3' end forward primer reverse primer annealing temperature PZ P
cDNA Synthesis from MOLT-4 Cells
second strand yield. 11. Add 0.5 ml PN buffer from Qiegen Nucleotide Removal Kit to the remaining reaction, and mix it ,load it into the spin column, and follow the pr℃edures comimg with the kit to purify the double strand DNA. 12. Elute the cDNA
Library cDNA Synthesis 1° cDNA Synthesis N.B: During 1° cDNA synthesis, all steps should be carried while wearing gloves and all solutions should either have been DEPC-treated or purchased specifically for use with RNA.
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