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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
Terra™ PCR Direct Polymerase Mix
- 供应商：
北京智杰方远
- 保存条件：
-20度
- 规格：
40 rxns
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文献和实验Creating a deletion by PCR splicing
Creating a deletion by PCR splicing Contributed by Dr.A.Gratchev I didn't want to place this in the methods section due to its simplicity. To delete a desired fragment from existing DNA fragment all you need is a pair
well in the PCR buffer. However, you must gel-purify the digested DNA. 4) For the second PCR step, you can start the PCR without the oligo I and II, then add the oligos after a few cycles. 5) Do not use Vent polymerase for mutagenesis work.
Single tube confirmation PCR protocol
Polymerase. - Add 35 µl of the PCR mix to each of the 20 PCR tubes that already contain the 15 µl of primers and template. 110 µl 5 µl 10 x Taq buffer(see below) 11 µl 0.5 µl 20 mM dNTP's (0.2 mM) 11 µl 0.5 µ
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