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文献和实验E.Z.N.Z.TM MicroElute RNA Desalting and Concentration protocol
. Discard the flow-through and the collection tube. 8. Elution of RNA. Transfer the column to a clean 1.5 ml microfuge tube (not supplied with kit) and pipet 15-30 ul of DEPC-treated water (supplied with kit) into the column. Make sure to add water
Dynabeads® Co-Immunoprecipitation Kit
acids (NA) after co-immunoprecipitation of DNA or RNA binding protein complexes 5. EB For the elution of the isolated protein complex, the buffer EB is supplied in the kit. However, for large scale co-immunoprecipitations
E.Z.N.A.® Total RNA Midi Kit Protocol for Eukaryotic Cells and Tissues
and add the entire mixture to the column. With the spin column inside the 15 ml collecting tube (supplied with kit), centrifuge at 4,000-5,000x g for 5 min at room temperature. Discard flow-through and proceed to step 4. 4. Wash column with RNA Wash
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