S1P1 stable cell line

Generation of a Stable Cell Line for Constitutive miRNA Expression

Here, a protocol is described to generate stable cell lines for constitutive expression of single cellular or viral miRNA precursors in absence of infection. The procedure comprises cloning of the precursor sequence, generation of the lentiviral expression vector

Robust Cell Line Development Using Meganucleases

Cell line development for protein production or for the screening of drug targets requires the reproducible and stable expression of transgenes. Such cell lines can be engineered with meganucleases, sequence-specific endonucleases

细胞系 cell line

　　 指从某初代培养开始通过连续培养所产生的所有细胞。包括从初代培养的细胞和细胞群所发展的一系列的细胞。如果培养的状态清楚，则在前面冠以有限继代性（ finite）或连续继代性（ continuous）。例如，人二倍体成纤细胞系属于前者， HeLa细胞系属后者。另外连续继代性细胞系也称为树立细胞系。