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EP4 Flash Aequorin Cell Line
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文献和实验Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
by sequence?specific nucleases in one?cell embryos provides an expedited mutagenesis approach in rodents. This technology has been recently established to create knockout and knockin mutants through sequence deletion or sequence insertion. This article
. (2001)Genome Res. 11, 3–11 65. Sedimentation field flow fractionation purification of immature neural cells from a human tumor neuroblastoma cell line. Lautrette, C. et al. (2003)J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.791, 149–160 66. Flow
Midi/Maxi Kit. 16. Standard gel electrophoresis reagents and apparatus. 2.2 Cell Culture and Processing Reagents 1. Cell line: human embryonic kidney (HEK) 293FT cell line. 2. Dulbecco’s Modifi ed Eagle’s Medium (DMEM). 3. 10 % fetal
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