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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
Tris(hydroxymethyl)aminomethane TRIS LAB
- 供应商:
北京智杰方远科技
- CAS号:
******
- 保存条件:
详见说明
- 规格:
****
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文献和实验Molecular Weight Determinations Using Polyacrylamide Gel Electrophoresis with Tris-Tricine Buffers
to the more mobile Tricine (N-tris[hydroxymethyl]-methylglycine) and by lowering the pH of the separating gel.
Reagents: ChIP sonication Buffer (1% Triton X-100,0.1% Deoxycholate,50 mM Tris 8.1,150 mM NaCl,5 mM EDTA):10 ml 10% Triton X-1001 ml 10% Deoxycholate5 ml 1 M Tris-Cl pH 8.11 ml 0.5 M EDTA3 ml 5 M NaCl80 ml WaterJust before use,add 10μl Aprotinin,10μ
RNase Protection Assay (Bowtell Lab)
buffer: 10mM Tris pH 7.5 5mM EDTA 300mM NaCl 40ug/ml RNAse A (Sigma) 2ug/ml RNAse T1 (Sigma) 11. Incubate at ___ � for ____min. Optimal times and temperatures vary for different probes. Overdigestion gives lots of smlall bands and underdigestion
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