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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
5X iSCRIPT RXN MIX (100)
- 供应商:
北京智杰方远
- 保存条件:
详见产品说明
- 规格:
**
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文献和实验-free water to 15 m l 3. Add 5x iScript Reaction Mix , 4 m l for a 20 m l rxn volume. 4. Add iScript RT , 1 m l. 5. Incubate complete reaction mix at following temperatures: a. 5 minutes at 25 ° C b.
in magnetic stand for 30 sec or longer to collect the beads. c) remove supernatent and wash 3X with 200 ul of 0.5X SSC d) finally resuspend the SA-PMPs in 100ul of 0.5X SSC for each 200 ul of beads originally removed - each 100 ul aliquot
For hybridization, make pre-mix of salts in a separate tube (will use 4 µl of pre mix/rxn): Pre-mix: 2 µl 5M NaCl 1.6 µl 0.5M PIPES, pH 7.0 0.2 µl 0.1M EDTA, pH 7.0 0.2 µl water 3. Add the following to your sample: 16 µl formamide 4 µl pre-mix salts
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