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- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
ISCRIPT SMPL PREP BUFFER,5X100
- 供应商：
北京智杰方远
- 保存条件：
详见产品说明
- 规格：
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
, during time series analyses or when screening transfor-mants for expression of a transformed gene. Under such circumstances, existing tech-niques are overly time consuming and yield more RNA than is necessary. The availability of a rapid RNA mini-prep
Preparation of protein extracts for western blot 1.Grow cells to mid-log (OD600 less or equal to 1.0). 2.Harvest about 5 OD's of cells in a 13X100 glass dispo tube (Use new tubes.Can be up to about 6 mls of culture). 3.Pellet 3-5 min.in Tomy,3K
Fast and reliable mini-prep RNA extraction from Neurospora crassa
otherwise. - Pulverize the mycelium in a mortar with liquid nitrogen - Transfer the powder into a mixture of 0.75 ml lysis buffer (0.6 M NaCl, 10 mM EDTA, 100 mM Tris HCl, pH 8.0, 4 SDS) and 0.75 ml phenol (saturated with 0.1 M Tris HCl, pH 8.0) in a 2 ml
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