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文献和实验Several important signaling processes depend on the tagging of cellular proteins with “polyubiquitin chains”—ubiquitin polymers whose building blocks are connected by isopeptide bonds between G76 of one ubiquitin and a specific lysine residue
Studies of the Ubiquitin Proteasome System
with Nonradioactive Tag (Biotin) Alternate Protocol 10: Generation of Tagged Ubiquitin Expression Plasmids Basic Protocol 18: Immunoblotting with Anti‐Ubiquitin Generation and Purification of Rabbit Anti‐Ubiquitin Antibodies Basic Protocol 19: Generation
Measuring Ubiquitin Conjugation in Cells
Protein ubiquitination is crucial to many diverse and critical functions of cells. Although it has been long known that conjugation of ubiquitin to proteins results in their destruction by the proteasome, recently it has become apparent
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