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文献和实验Western blotting using ECL reagent
REAGENTSECL Western blotting kit (Amersham Life Science; cat# RPN2108): contains second antibodies for both mouse and rabbit, substrate and milk blocker (the milk blocker is not normally used when using ruminant samples).Hybond ECL nitrocellulose
(All steps with agitation, unless done O/N at 4℃)►Rinse membrane once in TBS-T►Block the membrane with TBS-T/ 5% milk (or HIHS) for 30 min room►temp (can be left at 4℃ O/N here)►Rinse membrane briefly 2X with TBS-T and then once for 10 min with TBS
SDS-PAGE Western Blotting Protocols
Adapted from existing protocols by Vinh Pham Last modified: June 5, 2003. MATERIALS All materials required are mentioned within the respective sections below. PROCEDURE Part I – Sample Preparation 1. Collect
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