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文献和实验for routine analytical gels, and 1.5 mm spacers to run more sample. I usually use a comb that makes 20 wells.2. Solutions:I. 1.5 M Tris-HCl, pH 8.8II. 1 M Tris-HCl, pH 6.8III. 30% acrylamide/bisacrylamide (37.5:1). This solution can be bought commercially
.6. Load 10μl- 25μl(10μl for a 15 tooth mini comb) and then overlay samples with 1 x SDS running buffer. Empty wells should have 10-15μl sample buffer added and be overlayed as with the test samples so the gel runs straight. Flood the upper chamber
(SDS-PAGE). The most commonly used system is also called the Laemmli method after U.K. Laemmli, who was the first to publish a paper employing SDS-PAGE in a scientific study. SDS (also called lauryl sulfate) is an anionic detergent, meaning
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