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文献和实验RNA AND PROTEIN EXTRACTION FROM THE SAME SAMPLE
-HCl pH 8. Store in dark bottle at 4o C for 1-4 month 3. Phenol: Chloroform: Isoamyl alcohol (PCI) (25:24:1) (50 mL): Mix 25 mL of equilibrated saturated phenol, 24 mL of chloroform and 1 mL of isoamyl alcohol. Store
described below is for a 100 µl sample volume using 5 ml color reagent.It is sensitive to about 5 to 200 micrograms protein,depending on the dye quality.In assays using 5 ml color reagent prepared in lab,the sensitive range is closer to 5 to 100 µg protein
Bicinchoninic Acid (BCA) Protein Assay (Smith)
.The working solution is stable for 1 week and should be green. Assay 1.Prepare samples containing 0.2 to 50 micrograms protein in microliters. 2.Add 1 ml SWR to each 20 microliters sample and mix.Incubate 30 min.at 60 degrees C. 3.Cool the samples and read
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