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文献和实验temperature 45 minutes 8. Wash in water 10 minutes 9. Differentiate in Acid alcohol 10. Wash in water 10 minutes 1. Stain in Picro-sirius red 1 hour 12. Wash in water 13. Dehydrate, clear . Mount sections in DPX N.B. Wash quickly
Rapid Electrical Lysis of Bacterial Cells in a Microfluidic Device
. In this study we fabricated a simple microfluidic device using low-cost soft lithography with channel widths considerably larger than the cell size to avoid clogging and ensure stable performance, which was able to lyse green fluorescent protein (GFP)-expressing
of 7 x 2 x 0.5" and bottom dimensions of 7 x 2 x 0.25". 2) Recess the top plate in the center portion to 0.125" deep, leaving 0.950" per end for 1.375" wide x 0.3125" deep fluid reservoir. 3) Drill 1/16" wide x 0.625" long
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