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文献和实验Alcian green stain for cartilage
% ethanol, several hours to overnight each. 6. Clear by washing 1-3X with methyl salicylate. Alcian green stain: 0.1 g Alcian green 70 ml 100% ethanol 1 ml conc. HCl 29 ml dH2O (100 ml)
Purification of Plasmid from 50 ml-culture
1. Shake E. coli harboring plasmid at 37 C overnight in 50 ml of TB containing appropriate antibiotics. (when using ampicillin, addition of the antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of plasmids.)
Vybrant® DyeCycle Green and Orange Stains
material is supplied for approximately 200 fl ow cytometry assays based on a 1 mL test volume. Approximate fluorescence excitation/emission maxima: Vybrant® DyeCycle™ Green stain: 506/534 nm, bound to DNA; Vybrant® DyeCycle™ Orange stain: 519/563 nm
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