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文献和实验Chromatin IP (CHIP assay) This protocol has some minor modification to the protocol described in Strahl-Bolsinger S. et al. [1997, Gen & Dev 11, p83-93] and was obtained from Flick K. (The Scripps Research Institute).
Introduction Cross-linking Chromatin Immunoprecipitation (ChIP) has become a popular method to detect the in vivo binding of proteins to DNA. In general the protein/DNA complex is fixed with formaldehyde, a cross-linking agent
Yeast Chromatin Immunoprecipitation (ChIP)
Yeast Chromatin Immunoprecipitation (ChIP)Hahn LabJuly 2003Chromatin IP MethodDay 11.Start 5 ml o/n cultures of strains to be testedDay 21.Inoculate 50 ml of the desired media with a volume of a saturated o/n culture and grow o/n with shaking at 200
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