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文献和实验Protein Detection Using Reversible Metal Chelate Stains
on nitrocellulose, PVDF, and nylon membranes as well as in polyacrylamide gels. The metal complexes do not modify proteins, and are compatible with immunoblotting, lectin blotting, mass spectrometry, and Edman-based protein sequencing (13 –17 ). Metal chelate
Methods to Detect Protein Glutathionylation
and enzyme?mediated reactions. Protein glutathionylation is observed in response to oxidative or nitrosative stress and is redox?dependent, being readily reversible under reducing conditions. Such findings suggest that glutathionylation plays an important
Identification of Protein Interactions by Far Western Analysis
protein of interest cloned into an in vitro expression vector In vitro transcription/translation kit (Promega) 10 mCi/ml 35
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