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文献和实验Immunohistochemisty-Fluorescence Protocol
eukaryotic cells expressing the intra-cellular target cytokine protein. Stain cultured blood mononuclear cells or spleen cells that have been harvested, fixed and permeabilized at the peak of cytokine production after in vitro stimulation with strong
Immunohistochemistry: Fluorescence Protocol 2.0
Controls Positive staining controls Stain fixed, permeabilized cytokine-cDNA transfected eukaryotic cells expressing the intra-cellular target cytokine protein. Stain cultured blood mononuclear cells or spleen cells
HIGH RESOLUTION GENETIC FOOTPRINTING
9600 thermal cycler with thin-walled 0.2 ml tubes, and AmpliTaq polymerase from Perkin-Elmer. 6.) Materials for preparative agarose gel electrophoresis. We use the Quiaquick gel extraction kit (Qiagen) for all band excision steps, and the Quiaquick
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