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- 文献和实验
- 技术资料
- 抗体英文名:
alpha-Tubulin (11H10) Rabbit mAb
- 是否单克隆:
多克隆
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文献和实验7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
Immunofluorescent Localization of Tubulin
LEVEL II Materials Coverslip cultures of an appropriate monolayer cell line Phosphate buffered saline (PBS) Acetone/Methanol (absolute) in a 50:50 volume mixture Rabbit anti-tubulin (or other primary
the resulting pellets in 0.25 x V2 of cold PM buffer, incubate at on ice for 30 min and centrifuge again (steps 11-15). 18. The resulting cold supernatant is ~90% pure tubulin and is called twice-cycled tubulin
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