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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
NTA Agarose (25ml)
- 供应商:
北京智杰方远科技主要为科研领域提供分子生物学、细胞生物学、抗体等相关产品。公司主要代理:Invitrognen、SIGMA、Roche、Abcam、QIAGEN等品牌。我们以“团结诚信,求实创新,质量为本,服务第一”为经营理念。在生命科学领域不断探索,希望不断发展的智杰方远能成为您最佳的选择!
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文献和实验Purification of 6xHis epitope tagged proteins by Ni-NTA-Agarose His标签蛋白纯化
Low (“Low”)Imidazole Buffer 0.5L 100mM Imidizole 3.4g 5% glycerol 25ml 100% glycerol 50mM Tris-HCl (pH 7.9)50ml 0.5M Tris-HCl (pH 7.9) 0.1% Tween-20 0.5M 100% Tween-20 500mM NaCl50ml 5M NaCl dH2O Fill to 0.5L (start w/ 350ml) High (“High
10 mg/ml ethidium bromide To prepare 100 ml of a 0.7% agarose solution, measure 0.7 g agarose into a glass beaker or flask and add 100 ml 1X TBE or TAE. Microwave or stir on a hot plate until agarose is dissolved
A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
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