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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1
- 保存条件:
-20°c
- 供应商:
Merck
- 英文名:
KOD Hot Start Master Mix
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文献和实验(BD Biosciences / Clontech). SYBR Green PCR master mix, 200 reactions (Applied Biosystems). Optical tube and cap strips (Applied Biosystems). SuperScript
Single tube confirmation PCR protocol
. For example, add 5 µl of the Zymo solution from isolate #1 to PCR tubes 1-5, 5 µl of the Zymo solution from isolate #2 to PCR tubes 6-10 and so on. - Make a PCR master mix by combining: 638 µl water, 110 µl 10 x Taq Buffer, 11 µl NTP's, and 11 µl Taq
, temperatures, and amount of template DNA, may vary and must be individually determined. 1) Thaw primer solutions. Keep on ice after complete thawing, and mix well before use. 2) Mix the PCR Master Mix by vortexing briefly. It is important
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