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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
Merck
- 库存：
1
- 英文名：
5X ANTIGENPLUS BUFFER (pH 10)
- 保存条件：
2-8°c
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文献和实验CCMB buffer to make chemical competent cell
does not appear to be necessary Test competence (see below) Good cells should yield around 100 - 400 colonies 4. CCMB80 buffer 10 mM KOAc pH 7.0 (10 ml of a 1M stock/l) 80 mM CaCl2.2H2O (11.8 g/l) 20 mM MnCl2.4H2O (4.0 g/l) 10 mM MgCl2.6H2O (2.0 g/l) 10
TOP10 chemically competent cells
too much per tests on 1.23.07 --Meaganl 15:50, 25 January 2007 (EST) water to 1 liter autoclave or sterile filter Test pH adjustment by mixing 4 parts ligation buffer + 1 part 5x ligation adjustment buffer and checking pH
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
Steve HahnProtein Dilution Buffer 5ml20 mM Tris pH7.9 100 microliters 1M Tris 7.9150 mM KCl 0.75 ml 1 M KCl1 mM DTT 50 microliters 0.1 M DTT10% glycerol 1 ml 50% glycerol50 micrograms/ml BSA 2.5 microliters 100 mg/ml BSA3.1 ml H2OOptional: add Brij
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