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Single Read, w/ prep
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文献和实验Single Strand DNA Prep. for Sequencing
This protocol works well from a 5 ml starting culture or a 1 ml starting culture (adjust volume accordingly). Solutions 2X YT Media 16 g tryptone 10 g yeast extract 5 g NaCl 1 ml 1N NaOH up to 1 liter with Q Ampicillin Stock (1000X) 0.15 g
On the Accuracy of Short Read Mapping
The development of high-throughput sequencing technologies has revolutionized the way we study genomes and gene regulation. In a single experiment, millions of reads are produced. To gain knowledge from these experiments the first thing
RNA‐Seq Read Alignments with PALMapper
sites (d), acceptor splice sites (a), and edit operations for match/mismatch/gap on read (q). Each piece‐wise linear function is defined by the range of possible x values (length for h, splice‐site predictions for d and a, quality for q
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