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- 英文名:
QIAprep 96 Turbo Miniprep Kit (24)
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文献和实验from the handbook and annotated based on experience with the kit. Protocol: QIAprep Spin Miniprep Kit Using a Microcentrifuge This protocol is designed for purification of up to 20 μg of high-copy plasmid DNA from 1�5 ml overnight cultures of E. coli in LB
freeshotgun sequencing library from miniprep BAC DNA
Miniprep of BAC DNA 1.Inoculate 4 - 8 tubes of 3ml/tube LB with 15μg/ml chloramphenicol with BAC clone and grow oveernight. Miniprep with AutoGen 740 according to the protocol described in our Web page. DNA pellet
free shotgun sequencing library from miniprep BAC DNA
Miniprep of BAC DNA 1.Inoculate 4 - 8 tubes of 3ml/tube LB with 15μg/ml chloramphenicol with BAC clone and grow oveernight. Miniprep with AutoGen 740 according to the protocol described in our Web page. DNA pellet were resuspended in 265 μl TE
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