QIAprep Turbo Buffer Module 80

RNA-chromatin immunoprecipitations (RNA-ChIP) in mammalian cells

Lysis and sonication The cell pellet was resuspended in 200µl of Buffer A and placed on ice for 10 minutes. The crude nuclei fraction was pelleted by microcentrifugation for 5000 rpm for 5 minutes at 4ºC. The pellet was washed once in Buffer

生物芯片入门（二）：制作和结果分析

.A.L. Prep 96 Plasmid Kit（4x96）——高通量碱法纯化小量质粒DNA，只需60～75分钟即可纯化96个样品，需要96孔板离心机。 27191 QIAprep 96 Turbo Miniprep Kit（4x96）——高通量碱法纯化小量质粒DNA，采用真空抽滤的方法，只需45分钟即可纯化96个样品，无需96孔板离心机。质粒纯度高。 27781 QIAprep 96 M13 Kit（4x96）——高通量纯化M13单链DNA，采用真空抽滤的方法，只需45分钟即可纯化96个样品

Visualization of RNA Using Fluorescence Complementation Triggered by Aptamer‐Protein Interactions (RFAP) in Live Bacterial Cells

polymerase from Expand Long Template PCR System; Roche Diagnostics) and corresponding 10× buffer Pfu Turbo DNA Polymerase (Stratagene) and corresponding 10× buffer