- ￥665
- Merck-Calbiochem
- 614350-25MG
- 2025年09月17日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°c
- 供应商：
Merck
- 英文名：
Tomatidine HCl
- 库存：
1
- 规格：
25MG
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文献和实验Replication timing by density transfer
out of the microtitre well, I first add this 10x SCP to the well where the sample used to be, then apply it to the slot blot. Remove the membrane and UV crosslink immediately. I let cross-linked membranes sit in 6x SCP, 1% Sarkosyl, 0.1% BSA (diluted from Calbiochem's
, NaOH (Merck, cat. no. 1.06498) Potassium hydroxide, KOH (Merck, cat. no. 1.05033) Hydrochloric acid, HCl (Merck, cat. no. 1.09057) EQUIPMENT Dissection tools Standard cell culture equipment
ARABIDOPSIS THALIANA DNA ISOLATION PROCEDURE
the tissue and stir for 3 min., pour into Buchner funnel to remove ether, rinse with cold H2O. Add 300 ml of buffer A (1 M sucrose, 10 mM Tris-HCl (pH 7.2), 5 mM MgCl2 , 5 mM 2-mercaptoethanol and 400ug/ml ethidium bromide). Grind tissue with a Polytron
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