- ￥409
- Merck-Calbiochem
- 539137-1ML
- 2025年09月07日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
Merck
- 英文名：
Protease Inhibitor Cocktail V EDTA-Free
- 保存条件：
-20°c
- 库存：
1
- 规格：
1ML
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文献和实验Partial proteolysis with Staph. aureus V8 protease
mM Tris-HCl, pH 6.8, and 1mM EDTA. This is in Tilo's box 3, in the east Forma, #1. Generally, if you aren't sure how much enzyme to use, try 10, 50 and 100 nanograms of V8 protease. If V8 doesn't work for your protein, try papain
Partial proteolysis with Staph. aureus V8 protease
mM Tris-HCl, pH 6.8, and 1mM EDTA. This is in Tilo's box 3, in the east Forma, #1. Generally, if you aren't sure how much enzyme to use, try 10, 50 and 100 nanograms of V8 protease. If V8 doesn't work for your protein, try papain
for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin
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