- ￥473
- Merck-Calbiochem
- 521000-250MG
- 2025年09月05日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Phenylarsine Oxide
- 保存条件：
15-30°c
- 库存：
1
- 供应商：
Merck
- 规格：
250MG
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文献和实验Measurement of Arginine Metabolites: Regulators of Nitric Oxide Metabolism
is the substrate for nitric oxide synthases (NOS), and arginine availability regulates the production of nitric oxide. Through the activity of methyltransferases, arginine can be methylated to form monomethylarginine (NMMA), asymmetrical dimethylarginine (ADMA
. no. 621722) Creatine kinase (Roche, cat. no. 127566) ATP (AppliChem, cat. no. A1348) HEPES (Gerbu Biotechnik, cat. no. 1009) Magnesium acetate (Merck, cat. no. 1.05819) 1,4-DTT (Roth, cat. no. 6908.2)
ULTIMATE FREEZE-THAW LYSIS FOR MAMMALIAN CELLS
.Thaw sample on ice (thawing takes a few minutes).Vortex briefly.6.Repeat freeze-thaw cycle two more times.7.Thaw cell lysate.Add 250 U of Benzonase (Merck)to digest DNA.Mix.Incubate at room temperature for 10 minutes.8.Measure protein concentration
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