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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
15-30°c
- 库存:
1
- 供应商:
Merck
- 英文名:
Tunicamycin Streptomyces lysosuperficus
- 规格:
10MG
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文献和实验衣霉素 tunicamycin 为放线菌 Streptomyces lysosuperficus产生的核苷酸抗生素。可抑制具有被膜( envelope,含糖蛋白)的病毒的繁殖。作用机理是抑制合成糖蛋白糖链必需的拟脂(多萜醇, dolichol)中间产物的生成。又因为它可抑制在合成细菌细胞壁的肽聚糖或胞壁酸中必需的拟脂(细菌萜醇,多聚萜醇)中间产物的形成,所以显示对革兰氏阳性菌的抗菌性。最近,利用这种抑制拟脂中间产物形成的作用,来探索糖类复合物的活体合成及其功能。
Replication timing by comparative hybridization
degrees C. Both strains have the Zygosaccharomyces rouxii "R" recombinase gene behind the GAL promoter integrated at LEU2. Both strains are Leu+. Pronase : We use Calbiochem's Pronase (catalog #53702), which seems pretty clean--i.e., plates
Replication timing by density transfer
Calbiochem's Pronase (catalog #53702), which seems pretty clean--i.e., plates spread after Pronase treatment aren't contaminated--but I heard from someone who'd used Sigma's Pronase and had massive growth of Streptomyces. 20x SCP = 0.6 M Na2 HPO
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