手足口病检测试剂盒

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LIGHT DIAGNOSTICS TM Enterovirus 71 / Coxsackie A16 Reagent, ~25 tests, included in kit #3345

Description:
LIGHT DIAGNOSTICS? Enterovirus 71 / Coxsackie A16 Reagent, ~25 tests, included in kit #3345

Trade Name:
LIGHT DIAGNOSTICS
Chemicon (Millipore)

Product Overview:
Light Diagnostics Enterovirus 71 Monoclonal Antibody is a type-specific reagent intended for use in indirect immunofluorescence screening for the presumptive identification of enterovirus 71 obtained in cell culture and not intended for testing directly on human specimens.

For in vitro diagnostic use.

Test Principle:

Light Diagnostics Enterovirus 71 Monoclonal Antibody (MAb EV 71) can be used to identify an enterovirus 71 isolate in cell culture using an indirect immunofluorescence assay (IFA). The monoclonal antibody provided will bind to the type-specific enterovirus 71 isolate present in the cell culture slide. Unbound monoclonal antibody is removed by rinsing with phosphate buffered saline (PBS). A secondary FITC (fluorescein isothiocyanate) labeled antibody is then added which will bind to the antigen-antibody complex. Unbound secondary antibody is removed by rinsing with PBS. FITC exhibits an apple-green fluorescence when illuminated by ultraviolet light allowing visualization of the complex by microscopy. A positive result is indicated by specific cell fluorescence. Uninfected cells stain a dull red if Evans blue counterstain is used in the FITC-labeled secondary antibody or elsewhere in the procedure.


Key Applications:
Immunofluorescence

Usage Statement:
For in vitro Diagnostic Use
CE Mark

Kit or Assay Type:
Diagnostics Kits

Components:
Enterovirus 71 Monoclonal Antibody - (Catalog No. 3323)
One 1 mL dropper vial containing mouse IgG1 monoclonal antibody against enterovirus 71, protein stabilizer and 0.1 % sodium azide (preservative).