- ￥2713
- Merck-Calbiochem
- CC12-100UG
- 2026年06月28日
- Mouse
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- 文献和实验
- 技术资料
- 抗体英文名：
Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6)
- 宿主：
Mouse
- 库存：
1
- 供应商：
Merck
- 抗原来源：
Human等
- 是否单克隆：
0
- 保存条件：
2-8°c
- 规格：
100UG
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文献和实验Immunodetection of cyclin D1 and D2/D3 using flow cytometry
5-conjugated anti-mouse IgG (available from Jackson ImmunoResearch and Caltag) in a volume of 200 µ l. Incubate for 4 hours at 4°C Wash once with cold cyclin staining buffer and once with cold PBS/azide. Resuspend the cells in propidium iodide at 50 µ g/ml
. Test each tube w/H2O and discard.Add protein mixture to dialysis tube. Stir slowly in 100 mM Tris buffer or Binding buffer until pink color is out.Transfer protein mixture (Ab) to 50 ml Falcon tubes to determine volume.Dilute the mixture
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
the need for fluorescence compensation. This assay can be used with any instrument employing dual laser excitation, including the B-D Vantage, FACSCalibur and Coulter Elite. Materials Anti-cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326) or cyclin
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