Anti-Cytomegalovirus, clone 8B

Anti-Cytomegalovirus, clone 8B1.2 (ASR)

MAB8135

Human cytomegalovirus (HCMV) is a ubiquitous human pathogen that belongs to the herpes adenovirus family. The viral life cycle takes approximately seventy two hours (see figure below). After the initial fusion of the viral envelope with the plasma membrane of the cell, the encapisidated virus particle is released into the cytoplasm and within minutes, transits to the nucleus. Via active transport through the nuclear pore, the capsid gains entry and viral DNA is deposited. Viral gene expression then occurs in a temporally regulated manner, first with expression of the immediate early genes, followed by the early genes, then, after viral replication has commenced, the late genes. All of the immediate early proteins have been shown to be transactivators, with IE1-72 and IE2-86 being the most well characterized. These genes regulate the expression of factors required for virus replication.

CMV

8B1.2

Reacts with an immediate early non-structural antigen of 68-72 kDa. This antigen can be detected 1 hour after infection exhibiting an intranuclear staining pattern. This staining reaches a peak at 10-12 hours. This antigen persists and can be detected throughout the complete CMV infection cycle.

Immunofluorescence

ELISA 1:800

Western Blot: 1:200. Recognizes approximately a 70kDa band.

Immunofluorescence: 1:200-1:400.

Immunohistochemistry 1:200-1:800. See general protocols online for guidelines.

Works on paraffin embedded tissue sections.

FACS: See online protocol.

Final working dilutions and protocols must be determined by end user.

Human

IgG2a

Affinity purified immediate early antigen from MRC-5 cells infected with CMV AD169 (ATCC).

Cytomegalovirus Control Slides, Catalogue Number 5027-5

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Infectious Diseases

Infectious Diseases - Viral

This product is sold as an Analyte Specific Reagent (ASR), for which the analytical and performance characteristics have not been established. According to current FDA regulations, those products labeled ASR can only be used by high-complexity labs, including those regulated by CLIA, public health labs, VA hospitals, and other diagnostic manufactures. ANY search or review for information on these products is considered by MILLIPORE to be a request for information by the searcher, and subsequently an acceptance on the part of the searcher of the limitations of the use of these products.

Chemicon (Millipore)

This product is only available for sale in the United States and Canada.

Purified

Mouse

1 mg/mL

Monoclonal Antibody