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- 2025年09月22日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Xa CLEAVAGE CONTROL PROTEIN
- 保存条件：
-20°c
- 库存：
1
- 供应商：
Merck
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文献和实验Factor Xa Cleavage of MBP-Fusion protein
INTRODUCTION In many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The amount of factor Xa, temperature and length of incubation must be calibrated
Thrombin Cleavage of GST-Fusion protein
). Stop the reaction with PAGE-SDS sample buffer + 1mM PMSF and keep immediately at -20°C until use. Analyze PAGE-SDS gels versus a control of non-cleavaged protein. Longer incubation, more enzyme or higher temperature will increase cleavage; while lower
James Hardwick CNBr Cleavage Procedure
1. Immunoprecipitate the protein and run it on a preparative gel. CNBr cleavage must be done with protein transferred to a nitrocellulose filter. Neither Immobilon nor Nylon can substitute. IMPORTANT: Wash the NC 2X for 15 min
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