AssayPro—Human LDL ELISA Kit（低

Introduction
Human low-density lipoprotein (LDL) transports cholesterol from the liver to tissues where it is incorporated into cell membranes. LDL consists of a neutral lipid core surrounded by an outer shell of phospholipids and amphipathic apolipoprotein B-100, a ligand for hepatic clearance of plasma cholesterol via LDL receptors. LDL particles are formed from the lipolytic conversion of VLDL to IDL by lipoprotein lipase. LDL varies in size, shape, composition, and density, and comprise multiple subclasses (1 - 2). Elevation of small, dense LDL in plasma is associated with increased risk of myocardial infarction (3). Familial hypercholesterolemia, caused by a mutation in an LDL receptor gene, is an autosomal dominant disorder associated with elevated LDL levels and premature coronary atherosclerosis (4).

Principle of the Assay
The AssayMax Low-Density Lipoprotein (LDL) ELISA kit is designed for detection of LDL in plasma, serum, and cell culture supernatant. This assay employs a quantitative competitive enzyme immunoassay technique that measures LDL in less than 3 hours. A polyclonal antibody specific for LDL has been pre-coated onto a 96-well microplate with removable strips. LDL in standards and samples is competed with a biotinylated LDL sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.