AssayMax Human Prealbumin ELISA Kit 人前白蛋白PA酶联免疫试剂盒
Introduction Prealbumin (transthyretin) is a hepatic secretory protein thought to be important in the evaluation of nutritional deficiency and nutrition support (1). Prealbumin plays important physiological roles as a transporter of thyroxine and retinol-binding protein (2). Decreased prealbumin levels have been suggested to associate with malnutrition (3) and chronic kidney disease (4).
Principle of the Assay The AssayMax Human Prealbumin ELISA kit is designed for detection of human prealbumin in plasma, serum, urine, saliva, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures prealbumin in less than 4 hours. A polyclonal antibody specific for prealbumin has been pre-coated onto a microplate. Prealbumin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for prealbumin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.