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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名:
2-(β-Glucosyl)glycerol
- 库存:
1
- 供应商:
甄准生物
- CAS号:
10588-30-4
- 保存条件:
Hygroscopic, -20°C Freezer, Under Inert Atmosphere
- 规格:
5mg / 50mg
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文献和实验Triglyceride Lipase Assays Based on a Novel Fluorogenic Alkyldiacyl Glycerol Substrate
is characterized by an α/β hydrolase fold (1 ). Most lipases utilize the catalytic triad Ser-His-Asp, with the serine residue acting as the nucleophile in the initial step of carboxyl ester hydrolysis; this active site serine is contained within the Gly-Xaa-Ser
tris-Cl, PH 7.4, 0.25 mM DTT, 10 mM CaCl2;PAD 缓冲液:推荐配方为 0.1M tris-Cl, PH 7.4, 5 mM DTT, 10 mM CaCl2, 10 µg/µl aprotonin, 10 µg/µl leupeptin, 10 µg/µl pepstatin;4×Laemelli 样品缓冲液:推荐配方为 8% SDS,40% Glycerol,250 mM tris-Cl, PH 6.8,0.02% Bromophenol Blue
Blue Native Gel Electrophoresis
% glycerol, 25 mM BisTrsi-HCl, pH7.0 Mix gently Load 5.5 ul Protein marker Mr (kDa) Ferritin 880, 440 β-amylase 200 BSA 132, 66 β-lactoglobulin 35 Thyroglobulin (670kD) migrates at position close to ferritin dimer (880kD).
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