Triglyceride Lipase Assays Based on a Novel Fluorogenic Alkyldiacyl Glycerol Substrate
互联网
536
Lipases are responsible for extracellular degradation and intracellular metabolism of lipids in animals, plants, and microorganisms. The conformational motif of all lipases whose three-dimensional structure has been determined is characterized by an α/β hydrolase fold (1 ). Most lipases utilize the catalytic triad Ser-His-Asp, with the serine residue acting as the nucleophile in the initial step of carboxyl ester hydrolysis; this active site serine is contained within the Gly-Xaa-Ser-Xaa-Gly consensus sequence common to most lipases (1 ,2 ).
