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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
N-(5-Aminopentyl)maleimide Trifluoroacetate
- 库存：
1
- 供应商：
甄准生物
- CAS号：
222159-87-7
- 规格：
25mg / 250mg
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文献和实验Phycoerythrin conjugation protocol
(Molecular Probes) DTT Dithiothreitol NEM N-ethyl maleimide * 10 ml Sephadex G-10 columns (2) Separation column A long Sephacryl S-300 column
Proteomic Detection of Oxidized and Reduced Thiol Proteins in Cultured Cells
, the reduced thiols are first blocked with N -ethyl-maleimide, then the oxidized thiols reduced with dithiothreitol and labelled with 5-iodoacetamidofluorescein. The method is illustrated by treating Jurkat T-lymphoma cells with hydrogen peroxide and monitoring
ThiolDisulfide Redox Proteomics in Plant Research
with N -ethylmaleimide and methoxypolyethylene glycol maleimide-5000 (mPEG-Mal-5000). Both sets of methods provide experimental information that defines the redox proteome of the cell.
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